* Min nick distance (pegRNA::PE3):   * Max nick distance (pegRNA::PE3): 2. Select CRISPR Enzyme: Cas9-NGGCas9-NGCas9-SpRY 3. Number of top candidate sgRNAs to export in results table: 4. (Optional) Use a preselected sgRNA: 5. (Optional) Incorporate on-target/off-target predictions. (Cas9-NGG only)
* Upload Broad CRISPick or CRISPRscan results: Clear   * Use the wildtype sequence as input and report all possible sgRNAs.