Wildtype/reference sequence

Edited/desired sequence

1. Find PE3/PE3b secondary nicking sgRNAs:   Yes    No

    * Min nick distance (pegRNA::PE3): 
    * Max nick distance (pegRNA::PE3): 

2. Select CRISPR Enzyme:   

3. Number of top candidate sgRNAs to export in results table:   

4. (Optional) Use a preselected sgRNA:

5. (Optional) Incorporate on-target/off-target predictions. (Cas9-NGG only)

    * Upload Broad sgRNA finder or CRISPRscan results:    
    * Use the wildtype sequence as input and report all possible sgRNAs.